Lactoperoxidase-catalysed iodination of surface proteins on human melanoma cells
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چکیده
منابع مشابه
Basolateral plasma membranes of intestinal epithelial cells. Identification by lactoperoxidase-catalysed iodination and isolation after density perturbation with digitonin.
Lactoperoxidase-catalysed iodination was used to label intestinal epithelial cell sheets with 125I. The iodination was carried out under conditions that allowed little penetration of lactoperoxidase into the cells and membrane-bound 125I therefore provided an effective marker for following plasma-membrane fragments through subcellular-fractionation procedures. 2. After homogenization and isopyc...
متن کاملSolubilization and peptide "mapping" of a large external glycoprotein fraction labelled by lactoperoxidase-catalysed iodination of cultured fibroblasts.
A glycoprotein(s) of apparent molecular weight 220000-250000 can be labelled by lactoperoxidase-catalysed iodination of fibroblasts from a variety of species (Hynes, 1973 ; Hogg, 1974; Pearlstein & Waterfield, 1974; Yamada & Weston, 1974). The glycoprotein of hamster fibroblasts appears at the cell surface during interphase and is lost from the surface at mitosis (Hynes &Bye, 1974). Further, th...
متن کاملAccessibility of proteins in rat liver-free and membrane-bound ribosomes to lactoperoxidase-catalyzed iodination.
We have used lactoperoxidase-catalyzed iodination to study the topographical distribution of proteins in free and membrane-bound polysomes and in isolated ribosomal subunits. Free polysomes or rough microsomes were iodinated with 125I and then dissociated into 40 S and 60 S subunits by the use of puromycin/KCl. The separated subunits were iodinated with 13’1 and their protein composition analyz...
متن کاملLactoperoxidase-catalyzed iodination of horse cytochrome c:monoiodotyrosyl 74 cytochrome c.
Iodination of horse cytochrome c with the lactoperoxidase-hydrogen peroxide-iodide system results initially in the formation of the monoiodotyrosyl 74 derivative. This singly modified protein was obtained in pure form by ion exchange chromatography and preparative column electrophoresis. It shows an intact 695 nm absorption band, the midpoint potential of the native protein, a nuclear magnetic ...
متن کاملPlasmalemmal proteins of cultured vascular endothelial cells exhibit apical-basal polarity: analysis by surface-selective iodination
Vascular endothelium in vivo appears to function as a polarized epithelium. To determine whether cellular polarity exists at the level of the plasma membrane, we have examined cultured endothelial monolayers for evidence of differential distribution of externally disposed plasmalemmal proteins at apical and basal cell surfaces. Lactoperoxidase beads were used to selectively label the apical sur...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1978
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1978.170